Significant advances in corneal preservation have been made over the past decade. The introduction of chondroitin sulfate-containing media for use at 4 degrees C allows storage of corneas for up to ten days prior to transplantation. Organ culture techniques have also been improved with the addition of chondroitin sulfate. There has been an increase in our understanding of preservation using McCarey-Kaufman medium. Studies have been published that compare these methods and help the clinician decide which method to use based on objective data. Also, our understanding of existing methods of evaluating endothelial viability has increased and new methods have been developed. Finally, the acquired immunodeficiency syndrome is having an increasing effect on eye-banking and the supply of donor corneas.