CD99 regulates CXCL12-induced chemotaxis of human plasma cells

Minchan Gil; Hyo-Kyung Pak; A-Neum Lee; Seo-Jung Park; Yoonkyung Lee; Jin Roh; Hyunji Lee; Yoo-Sam Chung; Chan-Sik Park

doi:10.1016/j.imlet.2015.10.015

CD99 regulates CXCL12-induced chemotaxis of human plasma cells

Immunol Lett. 2015 Dec;168(2):329-36. doi: 10.1016/j.imlet.2015.10.015. Epub 2015 Nov 6.

Authors

Minchan Gil¹, Hyo-Kyung Pak², A-Neum Lee¹, Seo-Jung Park¹, Yoonkyung Lee¹, Jin Roh³, Hyunji Lee¹, Yoo-Sam Chung⁴, Chan-Sik Park⁵

Affiliations

¹ Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, South Korea.
² Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, South Korea; Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea.
³ Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea.
⁴ Department of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea.
⁵ Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, South Korea; Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea; Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea. Electronic address: csikpark@amc.seoul.kr.

PMID: 26522646
DOI: 10.1016/j.imlet.2015.10.015

Abstract

Migration of plasma cells (PCs) is crucial for the control of PC survival and antibody production and is controlled by chemokines, most importantly by CXCL12. This study investigated the role of CD99 in CXCL12-induced PC migration. Among B cell subsets in the tonsils, CD99 expression was highest in PCs. CD99 expression increased during in vitro differentiation of germinal center B cells and was highest in PCs. CD99 engagement reduced chemotactic migration of PCs toward CXCL12 and reduced extracellular signal-regulated kinase (ERK) activation by CXCL12. An ERK inhibitor reduced CXCL12-mediated chemotactic migration, which suggests that ERK has a critical role in migration. CD99 engagement did not influence apoptosis, differentiation, or antibody secretion of PCs. We propose a novel role of CD99 in PCs that suppresses ERK activation and chemotactic migration of these cells.

Keywords: CD99; Extracellular signal-regulated kinase; Migration; Plasma cell.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

12E7 Antigen
Animals
Antigens, CD / genetics
Antigens, CD / metabolism*
B-Lymphocytes / cytology
B-Lymphocytes / metabolism
Cell Adhesion Molecules / genetics
Cell Adhesion Molecules / metabolism*
Cell Differentiation / genetics
Cell Movement / drug effects
Cell Movement / genetics
Cells, Cultured
Chemokine CXCL12 / pharmacology*
Chemotaxis / drug effects*
Extracellular Signal-Regulated MAP Kinases / metabolism
Flow Cytometry
Gene Expression / drug effects
Gene Expression Profiling / methods
Germinal Center / cytology
Germinal Center / metabolism
Humans
Immunohistochemistry
L Cells
Mice
Plasma Cells / drug effects*
Plasma Cells / metabolism
Reverse Transcriptase Polymerase Chain Reaction

Substances

12E7 Antigen
Antigens, CD
CD99 protein, human
Cell Adhesion Molecules
Chemokine CXCL12
Extracellular Signal-Regulated MAP Kinases