A Review of Fluorescent Proteins for Use in Yeast

Maja Bialecka-Fornal; Tatyana Makushok; Susanne M Rafelski

doi:10.1007/978-1-4939-3145-3_21

A Review of Fluorescent Proteins for Use in Yeast

Methods Mol Biol. 2016:1369:309-46. doi: 10.1007/978-1-4939-3145-3_21.

Authors

Maja Bialecka-Fornal^{1

2}, Tatyana Makushok³, Susanne M Rafelski^{4

5}

Affiliations

¹ Department of Developmental and Cell Biology, Center for Complex Biological Systems, University of California, Irvine, CA, 92697, USA.
² Center for Complex Biological Systems, University of California, Irvine, CA, 92697, USA.
³ Department of Biochemistry and Biophysics, University of California, San Francisco, 600 16th Street, San Francisco, CA, 94158, USA.
⁴ Department of Developmental and Cell Biology, Center for Complex Biological Systems, University of California, Irvine, CA, 92697, USA. susanner@uci.edu.
⁵ Center for Complex Biological Systems, University of California, Irvine, CA, 92697, USA. susanner@uci.edu.

PMID: 26519321
DOI: 10.1007/978-1-4939-3145-3_21

Abstract

The field of fluorescent proteins (FPs) is constantly developing. The use of FPs changed the field of life sciences completely, starting a new era of direct observation and quantification of cellular processes. The broad spectrum of FPs (see Fig. 1) with a wide range of characteristics allows their use in many different experiments. This review discusses the use of FPs for imaging in budding yeast (Saccharomyces cerevisiae) and fission yeast Schizosaccharomyces pombe). The information included in this review is relevant for both species unless stated otherwise.

Keywords: Fluorescence microscopy; Fluorescent protein s; Protein tagg ing; Saccharomyces cerevisiae; Schizosaccharomyces pombe; Yeast.

Publication types

Review

MeSH terms

Databases, Factual
Gene Expression*
Genes, Reporter*
Genetic Vectors / genetics
Luminescent Proteins / genetics*
Luminescent Proteins / metabolism
Molecular Imaging / methods
Web Browser
Yeasts / genetics*
Yeasts / metabolism*

Substances

Luminescent Proteins