Crystallization and preliminary X-ray analysis of the human c-H-ras-oncogene product p21 complexed with GTP analogues

A Scherer; J John; R Linke; R S Goody; A Wittinghofer; E F Pai; K C Homes

doi:10.1016/0022-2836(89)90540-8

Crystallization and preliminary X-ray analysis of the human c-H-ras-oncogene product p21 complexed with GTP analogues

J Mol Biol. 1989 Mar 5;206(1):257-9. doi: 10.1016/0022-2836(89)90540-8.

Authors

A Scherer¹, J John, R Linke, R S Goody, A Wittinghofer, E F Pai, K C Homes

Affiliation

¹ Biophysik, Max-Planck-Institut für Medizinische Forschung, Heidelberg, F.R.G.

PMID: 2649686
DOI: 10.1016/0022-2836(89)90540-8

Abstract

The catalytic domain (amino acid residues 1 to 166) of the human ras-oncogene product p21 complexed with the GTP analogues beta,gamma-imido-GTP (GMPPNP), beta,gamma-methylene-GTP (GMPPCP), and guanosine-5'-(gamma-thiotriphosphate) (GTP gamma S) have been been crystallized. Crystals of the GMPPNP and GMPPCP complexes are well suited for high resolution X-ray crystallography. They belong to space group P3(1)21 (or its enantiomorph P3(2)21) with unit cell axes a=b=40.3 A and c = 162.2 A.

MeSH terms

Base Sequence
Crystallization
Genes, ras*
Guanosine Triphosphate / analogs & derivatives*
Humans
Macromolecular Substances
X-Ray Diffraction

Substances

Macromolecular Substances
Guanosine Triphosphate