Use of Whole-Genome Sequencing to Link Burkholderia pseudomallei from Air Sampling to Mediastinal Melioidosis, Australia

Emerg Infect Dis. 2015 Nov;21(11):2052-4. doi: 10.3201/eid2111.141802.

Abstract

The frequency with which melioidosis results from inhalation rather than percutaneous inoculation or ingestion is unknown. We recovered Burkholderia pseudomallei from air samples at the residence of a patient with presumptive inhalational melioidosis and used whole-genome sequencing to link the environmental bacteria to B. pseudomallei recovered from the patient.

Keywords: Australia; Burkholderia pseudomallei; bacteria; biothreat; genome; gram-negative bacterial infections; melioidosis; pathogen transmission; pneumonia; zoonoses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Air Microbiology*
  • Australia
  • Burkholderia pseudomallei / genetics*
  • Burkholderia pseudomallei / isolation & purification
  • Burkholderia pseudomallei / pathogenicity
  • Disease Transmission, Infectious*
  • Humans
  • Male
  • Melioidosis / etiology*
  • Melioidosis / genetics
  • Melioidosis / microbiology
  • Melioidosis / transmission
  • Middle Aged
  • Sequence Analysis, DNA / methods
  • Sequence Analysis, DNA / statistics & numerical data