1H, 15N, and 13C chemical shift assignments of cyanobacteriochrome NpR6012g4 in the red-absorbing dark state

Qinhong Yu; Sunghyuk Lim; Nathan C Rockwell; Shelley S Martin; J Clark Lagarias; James B Ames

doi:10.1007/s12104-015-9653-8

1H, 15N, and 13C chemical shift assignments of cyanobacteriochrome NpR6012g4 in the red-absorbing dark state

Biomol NMR Assign. 2016 Apr;10(1):139-42. doi: 10.1007/s12104-015-9653-8. Epub 2015 Oct 19.

Authors

Qinhong Yu¹, Sunghyuk Lim¹, Nathan C Rockwell², Shelley S Martin², J Clark Lagarias², James B Ames³

Affiliations

¹ Department of Chemistry, University of California, Davis, CA, 95616, USA.
² Department of Molecular and Cellular Biology, University of California, Davis, CA, 95616, USA.
³ Department of Chemistry, University of California, Davis, CA, 95616, USA. jbames@ucdavis.edu.

Abstract

Cyanobacteriochrome (CBCR) photosensory proteins are phytochrome homologs using bilin chromophores for light sensing across the visible spectrum. NpR6012g4 is a CBCR from Nostoc punctiforme that serves as a model for a widespread CBCR subfamily with red/green photocycles. We report NMR chemical shift assignments for both the protein backbone and side-chain resonances of the red-absorbing dark state of NpR6012g4 (BMRB no. 26582).

Keywords: CBCR; Cyanobacteria; NMR; Photoreceptor; Phytochrome; Tetrapyrrole.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Absorption, Radiation*
Amino Acid Sequence
Bacterial Proteins / chemistry*
Bacterial Proteins / metabolism
Color
Models, Molecular
Nostoc*
Nuclear Magnetic Resonance, Biomolecular*
Photoreceptors, Microbial / chemistry*
Photoreceptors, Microbial / metabolism
Protein Structure, Secondary

Substances

Bacterial Proteins
Photoreceptors, Microbial

Abstract

Publication types

MeSH terms

Substances

Grants and funding