Establishment and validation of a 384-well antibacterial assay amenable for high-throughput screening and combination testing

Arti Mishra; Svetlana V Dobritsa; Marie-Laure Crouch; John Rabenstein; Joycelyn Xiang Yi Lee; Saravanakumar Dhakshinamoorthy

doi:10.1016/j.mimet.2015.09.019

Establishment and validation of a 384-well antibacterial assay amenable for high-throughput screening and combination testing

J Microbiol Methods. 2015 Nov:118:173-5. doi: 10.1016/j.mimet.2015.09.019. Epub 2015 Oct 1.

Authors

Arti Mishra¹, Svetlana V Dobritsa², Marie-Laure Crouch³, John Rabenstein³, Joycelyn Xiang Yi Lee¹, Saravanakumar Dhakshinamoorthy⁴

Affiliations

¹ Biology and Pharmacology, Albany Molecular Research, Inc., The Galen, #05-01, 61 Science Park Road, 117525, Singapore.
² Biology and Pharmacology, Albany Molecular Research, Inc., The Galen, #05-01, 61 Science Park Road, 117525, Singapore; Lead Discovery, Albany Molecular Research, Inc., 22215 26th Avenue SE, Bothell, WA 98021, United States.
³ Lead Discovery, Albany Molecular Research, Inc., 22215 26th Avenue SE, Bothell, WA 98021, United States.
⁴ Biology and Pharmacology, Albany Molecular Research, Inc., The Galen, #05-01, 61 Science Park Road, 117525, Singapore. Electronic address: Saravanakumar.Dhakshinamoorthy@amriglobal.com.

PMID: 26432950
DOI: 10.1016/j.mimet.2015.09.019

Abstract

A 384-well-based antibacterial assay amenable for high-throughput screening and combination testing is described. The assay uses 100-500nL of test compounds and tolerates up to 2.5% dimethyl sulfoxide concentrations. It can be used for screening compound libraries and testing combinatory/synergistic/antagonistic effects of antibiotics, small molecules, and natural product extracts.

Keywords: Antibacterial assay; Combination testing; DMSO tolerance; HTS.

MeSH terms

Anti-Bacterial Agents / pharmacology*
Bacteria / drug effects*
Drug Evaluation, Preclinical / methods*
Drug Interactions*
High-Throughput Screening Assays*
Microbial Sensitivity Tests / methods

Substances

Anti-Bacterial Agents