Evaluation of an immunochromatographic assay for direct identification of thermostable direct hemolysin-producing Vibrio parahaemolyticus colonies on selective agar plates

Kentaro Kawatsu; Junko Sakata; Taro Yonekita; Yuko Kumeda

doi:10.1016/j.mimet.2015.09.020

Evaluation of an immunochromatographic assay for direct identification of thermostable direct hemolysin-producing Vibrio parahaemolyticus colonies on selective agar plates

J Microbiol Methods. 2015 Dec:119:4-6. doi: 10.1016/j.mimet.2015.09.020. Epub 2015 Oct 1.

Authors

Kentaro Kawatsu¹, Junko Sakata², Taro Yonekita³, Yuko Kumeda²

Affiliations

¹ Division of Bacteriology, Osaka Prefectural Institute of Public Health, 3-69, Nakamichi 1-chome, Higashinari-ku, Osaka 537-0025, Japan. Electronic address: kawatu@iph.pref.osaka.jp.
² Division of Bacteriology, Osaka Prefectural Institute of Public Health, 3-69, Nakamichi 1-chome, Higashinari-ku, Osaka 537-0025, Japan.
³ R&D Center, NH Foods Ltd., 3-3 Midorigahara, Tsukuba, Ibaraki 300-2646, Japan.

PMID: 26432103
DOI: 10.1016/j.mimet.2015.09.020

Abstract

We evaluated the utility of an immunochromatographic assay (NH IC TDH) in identifying thermostable direct hemolysin (TDH)-producing Vibrio parahaemolyticus colonies on selective agar plates. The sensitivity of the NH IC TDH assay was 100% (189 samples) and its specificity was 100% (41 samples) compared with the presence of tdh.

Keywords: Diarrhea; Food-borne pathogen; Immunochromatographic assay; TDH-producing Vibrio parahaemolyticus.

Publication types

Evaluation Study

MeSH terms

Bacterial Toxins / genetics
Bacterial Toxins / metabolism
Chromatography, Affinity / methods*
Hemolysin Proteins / genetics
Hemolysin Proteins / metabolism*
Humans
Vibrio Infections / microbiology*
Vibrio parahaemolyticus / genetics
Vibrio parahaemolyticus / isolation & purification*
Vibrio parahaemolyticus / metabolism

Substances

Bacterial Toxins
Hemolysin Proteins
thermostable direct hemolysin