Fmoc Solid-Phase Peptide Synthesis

Paul R Hansen; Alberto Oddo

doi:10.1007/978-1-4939-2999-3_5

Fmoc Solid-Phase Peptide Synthesis

Methods Mol Biol. 2015:1348:33-50. doi: 10.1007/978-1-4939-2999-3_5.

Authors

Paul R Hansen¹, Alberto Oddo²

Affiliations

¹ Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. prh@sund.ku.dk.
² Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

PMID: 26424261
DOI: 10.1007/978-1-4939-2999-3_5

Abstract

Synthetic peptides are important as drugs and in research. Currently, the method of choice for producing these compounds is solid-phase peptide synthesis. In this nonspecialist review, we describe the scope and limitations of Fmoc solid-phase peptide synthesis. Furthermore, we provide a detailed protocol for Fmoc peptide synthesis.

Keywords: Coupling reagents; Fmoc solid-phase synthesis; Linkers; Resins.

MeSH terms

Amino Acids* / chemistry
Fluorenes* / chemistry
Peptides / chemical synthesis*
Peptides / chemistry
Solid-Phase Synthesis Techniques*

Substances

Amino Acids
Fluorenes
N(alpha)-fluorenylmethyloxycarbonylamino acids
Peptides