Increased levels of serum and tissue miR-107 in human gastric cancer: Correlation with tumor hypoxia

Cancer Biomark. 2015;15(6):851-60. doi: 10.3233/CBM-150529.

Abstract

Background: MicroRNAs can function as oncogenes and tumor suppressor genes in human cancers. The expression of miR-107 is high in various types of solid tumors.

Objective: The aim of this study was to evaluate the tumor and serum level of miR-107 and its correlation to HIF-1α expression in gastric cancer patients.

Methods: Quantitative real-time PCR was used to analyze the expression of miR-107 and HIF-1α in 36 pairs of fresh gastric cancer and matched adjacent normal tissue specimens and the serum of these patients compared to age matched controls.

Results: The expression level of miR-107 was significantly higher in tumor tissues compared to adjacent normal tissue (p= 0.04). For serum, the expression level of miR-107 was significantly higher in gastric cancer patients than in age matched controls (p= 0.04). The correlation between tumor and serum expression of miR-107 with tumor hypoxia was found to be significant (p≤ 0.001).

Conclusion: The overexpression of miR-107 in tumors and serum of gastric cancer patients and its correlation with HIF-1α expression in tumor tissues was indicated that miR-107 may have a potential to use as a biomarker for detection of gastric cancer patients and hypoxia in gastric cancer tumor.

Keywords: Gastric cancer; hypoxia; miR-107.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / blood
  • Biomarkers, Tumor / genetics*
  • Case-Control Studies
  • Female
  • Follow-Up Studies
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Hypoxia*
  • Hypoxia-Inducible Factor 1, alpha Subunit / blood
  • Hypoxia-Inducible Factor 1, alpha Subunit / genetics*
  • Male
  • MicroRNAs / blood
  • MicroRNAs / genetics*
  • Middle Aged
  • Neoplasm Grading
  • Prognosis
  • ROC Curve
  • Real-Time Polymerase Chain Reaction
  • Stomach Neoplasms / blood
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / pathology

Substances

  • Biomarkers, Tumor
  • HIF1A protein, human
  • Hypoxia-Inducible Factor 1, alpha Subunit
  • MIRN107 microRNA, human
  • MicroRNAs