Paenibacillus faecis sp. nov., isolated from human faeces

Int J Syst Evol Microbiol. 2015 Dec;65(12):4621-4626. doi: 10.1099/ijsem.0.000622. Epub 2015 Sep 18.

Abstract

A spore-forming, rod-shaped Gram-strain-positive bacterium, strain 656.84T, was isolated from human faeces in 1984. It contained anteiso-C15 : 0 as the major cellular fatty acid, meso-diaminopimelic acid was found in the cell wall peptidoglycan, the polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and aminophospholipids as the major components, and the predominant menaquinone was MK-7. The DNA G+C content was 52.9 mol%. The results of comparative 16S rRNA gene sequence studies placed strain 656.84T within the genus Paenibacillus. Its closest phylogenetic relatives were Paenibacillus barengoltzii and Paenibacillus timonensis. Levels of DNA-DNA relatedness between strain 656.84T and Paenibacillus timonensis CIP 108005T and Paenibacillus barengoltzii CIP 109354T were 17.3 % and 36.8 %, respectively, indicating that strain 656.84T represents a distinct species. On the basis of phenotypic and genotypic results, strain 656.84T is considered to represent a novel species within the genus Paenibacillus, for which the name Paenibacillus faecis sp. nov. is proposed; the type strain is 656.84T ( = DSM 23593T = CIP 101062T).

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • Cell Wall / chemistry
  • DNA, Bacterial / genetics
  • Diaminopimelic Acid / chemistry
  • Fatty Acids / chemistry
  • Feces / microbiology*
  • France
  • Humans
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Paenibacillus / classification*
  • Paenibacillus / genetics
  • Paenibacillus / isolation & purification
  • Peptidoglycan / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / chemistry

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Peptidoglycan
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • Diaminopimelic Acid
  • menaquinone 7