High-quality permanent draft genome sequence of Bradyrhizobium sp. Ai1a-2; a microsymbiont of Andira inermis discovered in Costa Rica

Rui Tian; Matthew Parker; Rekha Seshadri; Tbk Reddy; Victor Markowitz; Natalia Ivanova; Amrita Pati; Tanja Woyke; Mohammed Baeshen; Nabih Baeshen; Nikos Kyrpides; Wayne Reeve

doi:10.1186/s40793-015-0007-z

High-quality permanent draft genome sequence of Bradyrhizobium sp. Ai1a-2; a microsymbiont of Andira inermis discovered in Costa Rica

Stand Genomic Sci. 2015 Jun 14:10:33. doi: 10.1186/s40793-015-0007-z. eCollection 2015.

Authors

Affiliations

¹ Centre for Rhizobium Studies, Murdoch University, Murdoch, Australia.
² Binghamton University, State University of New York, New York, USA.
³ DOE Joint Genome Institute, Walnut Creek, California USA.
⁴ Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California USA.
⁵ Center of Nanotechnology, King Abdulaziz University, Jeddah, Saudi Arabia ; Department of Biological Sciences, Faculty of Science, Jeddah University, Jeddah, Saudi Arabia.
⁶ Center of Nanotechnology, King Abdulaziz University, Jeddah, Saudi Arabia ; Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
⁷ DOE Joint Genome Institute, Walnut Creek, California USA ; Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.

Abstract

Bradyrhizobium sp. Ai1a-2 is is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an effective nitrogen fixing root nodule of Andira inermis collected from Tres Piedras in Costa Rica. In this report we describe, for the first time, the genome sequence information and annotation of this legume microsymbiont. The 9,029,266 bp genome has a GC content of 62.56% with 247 contigs arranged into 246 scaffolds. The assembled genome contains 8,482 protein-coding genes and 102 RNA-only encoding genes. This rhizobial genome was sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project proposal.

Keywords: Alphaproteobacteria; GEBA-RNB; Nitrogen fixation; Root-nodule bacteria; Symbiosis.