Copy Number Variation Analysis by Array Analysis of Single Cells Following Whole Genome Amplification

Eftychia Dimitriadou; Masoud Zamani Esteki; Joris Robert Vermeesch

doi:10.1007/978-1-4939-2990-0_14

Copy Number Variation Analysis by Array Analysis of Single Cells Following Whole Genome Amplification

Methods Mol Biol. 2015:1347:197-219. doi: 10.1007/978-1-4939-2990-0_14.

Authors

Eftychia Dimitriadou¹, Masoud Zamani Esteki², Joris Robert Vermeesch³

Affiliations

¹ Center for Human Genetics, Laboratory for Cytogenetics and Genome Research, KU Leuven, Leuven, 3000, Belgium.
² Center for Human Genetics, Laboratory of Reproductive Genomics, KU Leuven, Leuven, 3000, Belgium.
³ Center for Human Genetics, Laboratory for Cytogenetics and Genome Research, KU Leuven, Leuven, 3000, Belgium. Joris.vermeesch@uzleuven.be.

PMID: 26374319
DOI: 10.1007/978-1-4939-2990-0_14

Abstract

Whole genome amplification is required to ensure the availability of sufficient material for copy number variation analysis of a genome deriving from an individual cell. Here, we describe the protocols we use for copy number variation analysis of non-fixed single cells by array-based approaches following single-cell isolation and whole genome amplification. We are focusing on two alternative protocols, an isothermal and a PCR-based whole genome amplification method, followed by either comparative genome hybridization (aCGH) or SNP array analysis, respectively.

Keywords: Copy number variation analysis; Genomic microarrays; Multiple displacement amplification; Picoplex; Whole genome amplification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Comparative Genomic Hybridization / methods*
DNA Copy Number Variations*
Genome*
Genomics / methods
Nucleic Acid Amplification Techniques*
Oligonucleotide Array Sequence Analysis / methods*
Polymerase Chain Reaction / methods
Polymorphism, Single Nucleotide
Single-Cell Analysis / methods*