Oxidative stress and damage to erythrocytes in patients with chronic obstructive pulmonary disease--changes in ATPase and acetylcholinesterase activity

Biochem Cell Biol. 2015 Dec;93(6):574-80. doi: 10.1139/bcb-2015-0066. Epub 2015 Aug 18.

Abstract

The study indicates, for the first time, the changes in both ATPase and AChE activities in the membrane of red blood cells of patients diagnosed with COPD. Chronic obstructive pulmonary disease (COPD) is one of the most common and severe lung disorders. We examined the impact of COPD on redox balance and properties of the membrane of red blood cells. The study involved 30 patients with COPD and 18 healthy subjects. An increase in lipid peroxidation products and a decrease in the content of -SH groups in the membrane of red blood cells in patients with COPD were observed. Moreover, an increase in the activity of glutathione peroxidase and a decrease in superoxide dismutase, but not in catalase activity, were found as well. Significant changes in activities of erythrocyte membrane enzymes in COPD patients were also evident demonstrated by a considerably lowered ATPase activity and elevated AChE activity. Changes in the structure and function of red blood cells observed in COPD patients, together with changes in the activity of the key membrane enzymes (ATPases and AChE), can result from the imbalance of redox status of these cells due to extensive oxidative stress induced by COPD disease.

Keywords: ATPase; acetylcholinesterase; acétylcholinestérase; chronic obstructive pulmonary disease; erythrocytes; maladie pulmonaire obstructive chronique; oxidative stress; stress oxydant; superoxide dismutase; superoxyde dismutase; érythrocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholinesterase / blood
  • Acetylcholinesterase / metabolism*
  • Adenosine Triphosphatases / blood
  • Adenosine Triphosphatases / metabolism*
  • Biomarkers / blood
  • Biomarkers / metabolism
  • Down-Regulation
  • Erythrocyte Membrane / enzymology
  • Erythrocyte Membrane / pathology
  • Erythrocytes / enzymology*
  • Erythrocytes / pathology
  • GPI-Linked Proteins / blood
  • GPI-Linked Proteins / metabolism
  • Glutathione Peroxidase / blood
  • Glutathione Peroxidase / metabolism
  • Humans
  • Lipid Peroxidation*
  • Oxidative Stress*
  • Pulmonary Disease, Chronic Obstructive / blood
  • Pulmonary Disease, Chronic Obstructive / metabolism*
  • Pulmonary Disease, Chronic Obstructive / pathology
  • Pulmonary Disease, Chronic Obstructive / physiopathology
  • Severity of Illness Index
  • Sodium-Potassium-Exchanging ATPase / blood
  • Sodium-Potassium-Exchanging ATPase / metabolism
  • Sulfhydryl Compounds / blood
  • Sulfhydryl Compounds / metabolism
  • Superoxide Dismutase / blood
  • Superoxide Dismutase / metabolism
  • Up-Regulation

Substances

  • Biomarkers
  • GPI-Linked Proteins
  • Sulfhydryl Compounds
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • ACHE protein, human
  • Acetylcholinesterase
  • Adenosine Triphosphatases
  • Sodium-Potassium-Exchanging ATPase