A reagent for specific recognition of cysteine in aqueous buffer and in natural milk: imaging studies, enzymatic reaction and analysis of whey protein

Chem Commun (Camb). 2015 Nov 4;51(85):15592-5. doi: 10.1039/c5cc04876a.

Abstract

We report a new chemodosimetric probe () for specific recognition of cysteine (Cys) in aqueous buffer and in whey protein isolated from fresh cow's milk. Using this reagent we could develop a luminescence-based methodology for estimation of Cys released from a commercially available Cys-supplement drug by aminoacylase-1 in live cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amidohydrolases / chemistry*
  • Amidohydrolases / metabolism
  • Animals
  • Cattle
  • Cysteine / analysis*
  • Fluorescent Dyes / chemistry*
  • Hep G2 Cells
  • Humans
  • Indicators and Reagents / chemistry*
  • Milk / chemistry*
  • Molecular Structure
  • Water / chemistry
  • Whey Proteins / analysis*

Substances

  • Fluorescent Dyes
  • Indicators and Reagents
  • Whey Proteins
  • Water
  • Amidohydrolases
  • aminoacylase I
  • Cysteine