A new temperature-insensitive allele of the Arabidopsis AXR6/CUL1 locus derived from a missense mutation in the C-terminal RBX1 binding region

Plant Signal Behav. 2015;10(11):e1078956. doi: 10.1080/15592324.2015.1078956.

Abstract

We isolated a new recessive allele at the AUXIN RESISTANT6/CULLIN1 (AXR6/CUL1) locus, axr6-101, from an EMS-mutagenized population of Arabidopsis thaliana, the Landsberg erecta ecotype. axr6-101 is auxin resistant and semi-dwarf similar to the other recessive axr6 mutants. The axr6-101 phenotype is caused by the E716K substitution of the CUL1 protein, which is likely to affect its ability to bind to the C-terminal RING domain of RING-box 1 (RBX1). The previously reported allele of AXR6, cul1-7, is caused by a substitution at T510 that binds to the N-terminal β-strand of RBX1. Although cul1-7 shows temperature-sensitive phenotype, the axr6-101 phenotype is largely unaffected by temperature. axr6-101 may provide an important genetic resource for study of the structure-function relationship of the CUL1 protein.

Keywords: Arabidopsis thaliana; auxin resistance; auxin signaling; axr6; cullin; mutant.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles*
  • Arabidopsis / genetics*
  • Arabidopsis / growth & development*
  • Arabidopsis Proteins / metabolism*
  • Binding Sites
  • Carrier Proteins / metabolism*
  • Cullin Proteins / genetics*
  • Genetic Loci*
  • Indoleacetic Acids / pharmacology
  • Mutation, Missense / genetics*
  • Phenotype
  • Protein Binding / drug effects
  • Temperature*

Substances

  • Arabidopsis Proteins
  • Carrier Proteins
  • Cullin 1
  • Cullin Proteins
  • Indoleacetic Acids
  • RBX1 protein, Arabidopsis