In this study, the complete mitochondrial genome of Cryptolestes pusillus (GenBank accession number KT070713) was sequenced by long PCR and primer walking methods. The total length of mitochondrial DNA is 15 502 bp and contains 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and a A + T-rich region. The base composition of the genome is A (39.04%), T (37.07%), C (23.4%), and G (14.6%). Except for COI and ATP8 with TCC and ATC as start codon, respectively, the remaining protein-coding genes initiated with the three orthodox start codons. Two complete stop codons (TAA and TAG) and two incomplete stop codons (COIII stop with T and ND5 stop with TA) were used in the protein-coding genes. The A + T-rich region is located between 12s rRNA and tRNA(Ile) with the length of 859 bp. The phylogenetic relationships of Coleoptera species were constructed based on the nucleotide sequences of 13 protein-coding genes of mitogenome using the neighbor-joining method. The molecular-based phylogenetic analysis supported the traditional morphological classification on relationships within Coleoptera species.
Keywords: Cryptolestes pusillus; Laemophloeidae; mitochondrial genome.