Gaining insight into the catalysis by GH20 lacto-N-biosidase using small molecule inhibitors and structural analysis

Mitchell Hattie; Tasuku Ito; Aleksandra W Debowski; Takatoshi Arakawa; Takane Katayama; Kenji Yamamoto; Shinya Fushinobu; Keith A Stubbs

doi:10.1039/c5cc05494j

Gaining insight into the catalysis by GH20 lacto-N-biosidase using small molecule inhibitors and structural analysis

Chem Commun (Camb). 2015 Oct 18;51(81):15008-11. doi: 10.1039/c5cc05494j.

Authors

Mitchell Hattie¹, Tasuku Ito, Aleksandra W Debowski, Takatoshi Arakawa, Takane Katayama, Kenji Yamamoto, Shinya Fushinobu, Keith A Stubbs

Affiliation

¹ School of Chemistry and Biochemistry, The University of Western Australia, Crawley, WA 6009, Australia. keith.stubbs@uwa.edu.au.

PMID: 26312778
DOI: 10.1039/c5cc05494j

Abstract

The synthesis of potent inhibitors for lacto-N-biosidases and X-ray structural characterization of these compounds in complex with BbLNBase is described.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bifidobacterium / enzymology
Biocatalysis*
Crystallography, X-Ray
Dose-Response Relationship, Drug
Enzyme Inhibitors / chemical synthesis
Enzyme Inhibitors / chemistry
Enzyme Inhibitors / pharmacology*
Glycoside Hydrolases / antagonists & inhibitors*
Glycoside Hydrolases / metabolism
Models, Molecular
Molecular Structure
Small Molecule Libraries / chemical synthesis
Small Molecule Libraries / chemistry
Small Molecule Libraries / pharmacology*
Structure-Activity Relationship

Substances

Enzyme Inhibitors
Small Molecule Libraries
Glycoside Hydrolases
lacto-N-biosidase