Stepped gradients on polymeric monolithic columns by photoinitiated grafting

Sinéad Currivan; Damian Connolly; Brett Paull

doi:10.1002/jssc.201500776

Stepped gradients on polymeric monolithic columns by photoinitiated grafting

J Sep Sci. 2015 Nov;38(21):3795-802. doi: 10.1002/jssc.201500776.

Authors

Sinéad Currivan¹, Damian Connolly², Brett Paull^{1

3}

Affiliations

¹ Australian Centre for Research on Separation Science, School of Physical Sciences, University of Tasmania, Sandy Bay, Hobart, Tasmania, Australia.
² Pharmaceutical and Molecular Biotechnology Research Centre, Department of Science, Waterford Institute of Technology, Waterford, Ireland.
³ ARC Centre of Excellence for Electromaterials Science, School of Physical Sciences, University of Tasmania, Sandy Bay, Hobart, Tasmania, Australia.

PMID: 26311291
DOI: 10.1002/jssc.201500776

Abstract

The surface of polymethacrylate monoliths was functionalized by a post-polymerization modification, by means of a novel photo-initiated graft procedure where the charged monomer, sulfopropyl methacrylate, was controllably grafted stepwise, i.e. with incremental graft energies. The grafting approach was optimized using scanning capacitively coupled contactless conductivity detection. The effect of the localized ion exchange capacity and resultant gradient stationary phase upon ion-exchange chromatographic retention, selectivity, and performance was investigated, and compared to a homogeneously grafted (isotropic) column. The gradient column provided reduced peak widths at half height for both cationic analytes, with a reduction of 34 and 33%, respectively, when compared to the isotropic column.

Keywords: Capacitively coupled contactless conductivity detection; Cation exchange; Gradient; Grafting; Polymer monoliths.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cation Exchange Resins
Chromatography, Ion Exchange / instrumentation*
Polymers / chemistry*

Substances

Cation Exchange Resins
Polymers