Applications of Lipid Nanodiscs for the Study of Membrane Proteins by Surface Plasmon Resonance

Curr Protoc Protein Sci. 2015 Aug 3:81:29.13.1-29.13.16. doi: 10.1002/0471140864.ps2913s81.

Abstract

Methods for the initial steps of surface plasmon resonance analysis of membrane proteins incorporated in lipid nanodiscs are described. Several types of Biacore sensor chips are available and require distinct strategies to immobilize proteonanodiscs on the chip surface. The procedures for immobilization on three of these chips (NTA, antibody coupled CM5, and L1) are described in this unit and results are demonstrated for a model system with cytochrome P4503A4 (CYP3A4) in nanodiscs binding to a polyclonal anti-CYP3A4 antibody. Advantages and disadvantages of each chip type are considered.

Keywords: antibody-receptor binding; biosensor; lipid nanodisc; membrane protein; surface plasmon resonance binding analysis.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Antibodies, Immobilized / chemistry
  • Biosensing Techniques
  • Lipids / chemistry*
  • Membrane Proteins / chemistry*
  • Nanostructures / chemistry*
  • Surface Plasmon Resonance / methods*

Substances

  • Antibodies, Immobilized
  • Lipids
  • Membrane Proteins