Recent advances in genome editing and creation of genetically modified pigs

James R Butler; Joseph M Ladowski; Gregory R Martens; Matthew Tector; A Joseph Tector

doi:10.1016/j.ijsu.2015.07.684

Recent advances in genome editing and creation of genetically modified pigs

Int J Surg. 2015 Nov;23(Pt B):217-222. doi: 10.1016/j.ijsu.2015.07.684. Epub 2015 Jul 29.

Authors

James R Butler¹, Joseph M Ladowski¹, Gregory R Martens¹, Matthew Tector¹, A Joseph Tector²

Affiliations

¹ Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USA.
² Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USA. Electronic address: jtector@iupui.edu.

PMID: 26231992
DOI: 10.1016/j.ijsu.2015.07.684

Abstract

The field of xenotransplantation is benefiting greatly from recent advances in genetic engineering. The efficiency and pace with which new model animals are being created has dramatically sped progress towards clinical relevance. Endonuclease-driven genome editing now allows for the efficient generation of targeted genetic alterations. Herein we review the available methods of genetic engineering that have been successfully employed to create genetically modified pigs.

Keywords: Endonuclease; Homologous recombination; Off-target; TALEN CRISPR/Cas9; Zinc finger nuclease.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Animals, Genetically Modified / genetics*
Genetic Engineering / methods*
Genome*
Swine / genetics*
Transplantation, Heterologous