Clonogrouping, a Rapid Multiplex PCR Method for Identification of Major Clones of Listeria monocytogenes

J Clin Microbiol. 2015 Oct;53(10):3355-8. doi: 10.1128/JCM.00738-15. Epub 2015 Jul 29.

Abstract

Three multiplex PCR assays were developed to identify the 11 most common Listeria monocytogenes clones in clinical and food samples; 270 (95.7%) of 282 strains of serogroups IVb, IIb, IIa, and IIc were identified accurately. This novel tool is a rapid and efficient alternative to multilocus sequence typing for identification of L. monocytogenes clones.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Food Microbiology
  • Genotype*
  • Humans
  • Listeria monocytogenes / classification*
  • Listeria monocytogenes / genetics*
  • Listeria monocytogenes / isolation & purification
  • Listeriosis / diagnosis
  • Listeriosis / microbiology
  • Molecular Typing / methods*
  • Multiplex Polymerase Chain Reaction / methods*
  • Time Factors