A species of the genus Glycyrrhiza, G. eurycarpa P. C. Li recently reported as a new species growing in Gansu Province and Xinjiang Autonomous Region has not been studied before on its chemical constituents. This paper reports the isolation and chemical elucidation of four flavonoid glycosides from this species collected in Jinta County, Gansu Province. On the basis of physical and chemical constants and spectroscopic data (UV, IR, MS, 1HNMR and 13CNMR), compound I was confirmed as a new isoflavone diglycoside. Its structure was elucidated as formononetin-7-O[-D-apio-beta-D-furanosyl(1----2)]-beta-D-gl ucopyranoside. The new compound was named glycyroside. The other three components (II-IV) were identified as liquiritin, isoliquiritin and schaftoside, respectively.