A Luminogen with Aggregation-Induced Emission Characteristics for Wash-Free Bacterial Imaging, High-Throughput Antibiotics Screening and Bacterial Susceptibility Evaluation

Engui Zhao; Yilong Chen; Sijie Chen; Haiqin Deng; Chen Gui; Chris W T Leung; Yuning Hong; Jacky W Y Lam; Ben Zhong Tang

doi:10.1002/adma.201501972

A Luminogen with Aggregation-Induced Emission Characteristics for Wash-Free Bacterial Imaging, High-Throughput Antibiotics Screening and Bacterial Susceptibility Evaluation

Adv Mater. 2015 Sep 2;27(33):4931-7. doi: 10.1002/adma.201501972. Epub 2015 Jul 14.

Authors

Engui Zhao^{1

2}, Yilong Chen^{1

2}, Sijie Chen^{1

2}, Haiqin Deng^{1

2}, Chen Gui^{1

2}, Chris W T Leung^{1

2}, Yuning Hong^{1

2}, Jacky W Y Lam^{1

2}, Ben Zhong Tang^{1

2

3}

Affiliations

¹ HKUST Shenzhen Research Institute, Nanshan, Shenzhen, 518057, China.
² Division of Biomedical Engineering, Department of Chemistry, Institute for Advanced Study, Division of Life Science, State Key Laboratory of Molecular Neuroscience and Institute of Molecular Functional Materials, The Hong Kong University of Science & Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China.
³ Guangdong Innovative Research Team, SCUT-HKUST Joint Research Laboratory, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology (SCUT), Guangzhou, 510640, China.

PMID: 26179636
DOI: 10.1002/adma.201501972

Abstract

A luminogen with aggregation-induced emission characteristics is reported for bacterial imaging and antibiotics screening studies. The luminogen can light up bacteria in a wash-free manner, which simplifies the imaging process and increases its accuracy in bacterial detection. It can also be applied to high-throughput screening of antibiotics and fast evaluation of bacterial susceptibility, giving reliable results in less than 5 h.

Keywords: aggregation-induced emission; antibiotics screening; bacterial imaging; bacterial susceptibility; high-throughput.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology*
Drug Evaluation, Preclinical
Escherichia coli / drug effects*
High-Throughput Screening Assays*
Microscopy, Fluorescence*
Staphylococcus epidermidis / drug effects*

Substances

Anti-Bacterial Agents