Collectin CL-LK Is a Novel Soluble Pattern Recognition Receptor for Mycobacterium tuberculosis

PLoS One. 2015 Jul 14;10(7):e0132692. doi: 10.1371/journal.pone.0132692. eCollection 2015.

Abstract

Understanding the molecular components of immune recognition of the tuberculosis (TB) bacillus, Mycobacterium tuberculosis, can help designing novel strategies to combat TB. Here, we identify collectin CL-LK as a novel soluble C-type lectin able to bind M. tuberculosis, and characterize mycobacterial mannose-capped lipoarabinomannan as a primary ligand for CL-LK. Mice deficient in CL-K1, one of the CL-LK subunits, do not display altered susceptibility to M. tuberculosis. However, we found that the amount of CL-LK in the serum of patients with active TB is reduced, compared to that in controls, and correlates inversely to the magnitude of the immune response to the pathogen. These findings indicate that CL-LK might be of interest for future diagnostic and treatment monitoring purposes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Case-Control Studies
  • Collectins / blood
  • Collectins / deficiency
  • Collectins / genetics
  • Collectins / immunology*
  • Female
  • Humans
  • In Vitro Techniques
  • Ligands
  • Lipopolysaccharides / immunology
  • Lipopolysaccharides / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mycobacterium tuberculosis / immunology*
  • Mycobacterium tuberculosis / pathogenicity
  • Receptors, Pattern Recognition / metabolism*
  • Tuberculosis, Pulmonary / blood
  • Tuberculosis, Pulmonary / immunology

Substances

  • Colec11 protein, human
  • Collectins
  • Ligands
  • Lipopolysaccharides
  • Receptors, Pattern Recognition
  • lipoarabinomannan

Grants and funding

This work was supported by Centre National de la Recherche Scientifique (CNRS), University of Toulouse, Agence Nationale de la Recherche (ANR)/Programme d’Investissements d’Avenir (PIA grant number ANR-11-EQUIPEX-0003) and ANR-12-BSV3-0002 B-TB grant, French Ministry of Higher Education and Research (fellowship to A.T.), Fondation pour la Recherche Médicale (FRM fellowships to G.L.V., A.B. and C.D.), European Union (NEWTBVAC project, project n° 241745), and Fondation Bettencourt-Schueller. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.