Expression of Adiponectin Receptors on Peripheral Blood Leukocytes of Hypertensive Children Is Associated with the Severity of Hypertension

Biomed Res Int. 2015:2015:742646. doi: 10.1155/2015/742646. Epub 2015 Jun 4.

Abstract

The aim of the study was to find out whether peripheral blood leukocyte adiponectin receptors 1 and 2 (AdipoR1, AdipoR2) protein expression patterns (flow cytometry) differ between the primary hypertension children (n = 57) and healthy controls (n = 19) and if their expression levels are related to selected clinical parameters. The group of 26 patients [AdipoR(-)] showed lower and the group of 31 patients [AdipoR(+)] showed higher AdipoRs protein expression than the control and each other (P < 0.01 for neutrophils, P < 0.05 for monocytes). The AdipoR(+) leukocytes expressed higher AdipoR1 mRNA levels (RT-PCR) than AdipoR(-) ones and controls (P = 0.022 and P = 0.007, resp.). Despite greater BMI, the AdipoR(-) patients had unchanged serum adiponectin levels. In contrast, AdipoR(+) patients had lower serum adiponectin concentrations than the AdipoR(-) ones and controls (P < 0.001). The AdipoR(+) patients had higher blood pressure (P = 0.042) and greater carotid intima-media thickness (P = 0.017) than the AdipoR(-) ones. The stage of hypertension was associated with increased neutrophil but not monocyte AdipoR1 density (AdipoR1 MFI) (P < 0.05). Severe ambulatory hypertension was presented more often in AdipoR(+) patients than in AdipoR(-) ones (51.6% versus 26.9%, resp.; P < 0.01). In conclusion, neutrophil AdipoRs upregulation was associated with early stages of vascular injury, hypertension severity, and low serum levels of adiponectin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adiponectin / blood*
  • Adolescent
  • Child
  • Essential Hypertension
  • Female
  • Gene Expression Regulation
  • Humans
  • Hypertension / blood*
  • Hypertension / genetics
  • Hypertension / pathology
  • Insulin Resistance / genetics
  • Leukocytes / metabolism
  • Leukocytes / pathology
  • Male
  • Neutrophils / metabolism
  • Neutrophils / pathology
  • RNA, Messenger / blood
  • Receptors, Adiponectin / blood*
  • Receptors, Adiponectin / genetics

Substances

  • ADIPOQ protein, human
  • ADIPOR1 protein, human
  • ADIPOR2 protein, human
  • Adiponectin
  • RNA, Messenger
  • Receptors, Adiponectin