Surveillance of adult Aedes mosquitoes in Selangor, Malaysia

Trop Med Int Health. 2015 Oct;20(10):1271-80. doi: 10.1111/tmi.12555. Epub 2015 Jul 14.

Abstract

Objective: To determine the effectiveness of using sticky traps and the NS1 dengue antigen kit for the surveillance of Aedes mosquitoes for dengue control.

Methods: Apartments were selected in a dengue-endemic area, and sticky traps were set to capture adult Aedes mosquitoes. NS1 dengue antigen kit was used to detect dengue antigen in mosquitoes, and positive mosquitoes were serotyped using real-time RT-PCR.

Results: The sticky traps were effective in capturing Aedes aegypti, and a minimum of three traps per floor was sufficient. Multiple serotypes were found in individual mosquitoes.

Conclusion: The sticky trap and the NS1 dengue antigen test kit can be used as surveillance tool in dengue control programmes. This proactive method will be better suited for control programmes than current reactive methods.

Keywords: Aedes aegypti; Dengue; Kit de test de l'antigène NS1; Malaisie; Malasia; Malaysia; NS1 antigen test kit; dengue; kit de detección del antígeno NS1; piège collant; sticky trap; surveillance; trampas adhesivas; vigilancia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aedes / pathogenicity*
  • Animals
  • Dengue / epidemiology
  • Dengue / prevention & control*
  • Dengue Virus / isolation & purification
  • Dengue Virus / pathogenicity*
  • Feasibility Studies
  • Insect Vectors / pathogenicity*
  • Malaysia / epidemiology
  • Pilot Projects
  • Polymerase Chain Reaction / methods
  • RNA, Viral / isolation & purification
  • Specimen Handling / methods*

Substances

  • RNA, Viral