Illumina-based analysis the microbial diversity associated with Thalassia hemprichii in Xincun Bay, South China Sea

Ecotoxicology. 2015 Oct;24(7-8):1548-56. doi: 10.1007/s10646-015-1511-z. Epub 2015 Jun 20.

Abstract

In order to increase our understanding of the microbial diversity associated with seagrass Thalassia hemprichii in Xincun Bay, South China Sea, 16S rRNA gene was identified by highthrough sequencing method. Bacteria associated with seagrass T. hemprichii belonged to 37 phyla, 99 classes. The diversity of bacteria associated with seagrass was similar among the geographically linked coastal locations of Xincun Bay. Proteobacteria was the dominant bacteria and the α-proteobacteria had adapted to the seagrass ecological niche. As well, α-proteobacteria and Pseudomonadales were associated microflora in seagrass meadows, but the interaction between the bacteria and plant is needed to further research. Burkholderiales and Verrucomicrobiae indicated the influence of the bay from anthropogenic activities. Further, Cyanobacteria could imply the difference of the nutrient conditions in the sites. γ-proteobacteria, Desulfobacterales and Pirellulales played a role in the cycle of sulfur, organic mineralization and meadow ecosystem, respectively. In addition, the less abundance bacteria species have key functions in the seagrass meadows, but there is lack knowledge of the interaction of the seagrass and less abundance bacteria species. Microbial communities can response to surroundings and play key functions in the biochemical cycle.

Keywords: Cyanobacteria; Microbial communities; South China Sea; Thalassia hemprichii; Xincun Bay; α-Proteobacteria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / classification*
  • Bacteria / genetics
  • Bays / microbiology*
  • China
  • DNA, Bacterial / genetics
  • High-Throughput Nucleotide Sequencing
  • Hydrocharitaceae / microbiology*
  • Microbiota*
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA

Substances

  • DNA, Bacterial
  • RNA, Ribosomal, 16S