In vitro gene expression within membrane-free coacervate protocells

Chem Commun (Camb). 2015 Jul 21;51(57):11429-32. doi: 10.1039/c5cc04220h.

Abstract

Cell-free gene expression of a fluorescent protein (mCherry) is demonstrated within the molecularly crowded matrix of a polysaccharide/polypeptide coacervate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Artificial Cells / chemistry
  • Artificial Cells / metabolism*
  • Biomimetics
  • Dextrans / chemistry
  • Gene Expression*
  • Luminescent Proteins / genetics*
  • Plasmids / genetics
  • Polylysine / chemistry
  • Red Fluorescent Protein

Substances

  • Dextrans
  • Luminescent Proteins
  • Polylysine