Interconvertible hairpin structure found in the replication origin of phage G4 single-stranded DNA

I Hirao; Y Nishimura; M Ishida; K Watanabe; K Miura

Interconvertible hairpin structure found in the replication origin of phage G4 single-stranded DNA

Nucleic Acids Symp Ser. 1989:(21):13-4.

Authors

I Hirao¹, Y Nishimura, M Ishida, K Watanabe, K Miura

Affiliation

¹ Department of Industrial Chemistry, Faculty of Engineering, University of Tokyo, Japan.

PMID: 2608461

Abstract

We found a synthetic GCGAAAGC fragment with a mobility greater than that of other oligodeoxyribonucleotides in gel electrophoresis to take on a stable hairpin structure possessing two terminal G-C base pairs. The GCGAAAGC sequence is also found in the replication origin of phage G4 single-stranded DNA, but the hairpin structure originally proposed differs from that of the GCGAAAGC fragment we have studied. Possibility of rearrangement of the secondary structure in the replication origin of phage G4 was examined in relation to its replication initiation mechanism.

MeSH terms

Bacteriophages / genetics*
Base Sequence
DNA, Single-Stranded*
DNA, Viral*
Molecular Sequence Data
Nucleic Acid Conformation
Replicon*

Substances

DNA, Single-Stranded
DNA, Viral