The dependence of rotating frame spin-lattice relaxation, T1 rho on locking field frequency, f1, was measured for phantom materials and human breast tissues. These data were used to predict the relative signal strengths obtainable in a spin-locking imaging sequence. This imaging sequence was implemented on a 0.15-T imaging system and measurements of phantom and tissue signal strength for various imaging parameters agreed with predicted signal strengths. Compared to T1 and T2, T1 rho appears to have unique capability to distinguish tumor from normal fat and fibrous breast tissues. The applications of T1 rho to tissue characterization and imaging at high static field strengths are discussed.