Characterization of Whole Body Cholesterol Fluxes in the Mouse

Gemma Brufau; Albert K Groen

doi:10.1002/9780470942390.mo110118

Characterization of Whole Body Cholesterol Fluxes in the Mouse

Curr Protoc Mouse Biol. 2011 Dec 1;1(4):413-27. doi: 10.1002/9780470942390.mo110118.

Authors

Gemma Brufau¹, Albert K Groen^{1

2}

Affiliations

¹ Department of Pediatrics, Center for Liver, Digestive, and Metabolic Diseases, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
² Department of Laboratory Medicine, Center for Liver, Digestive, and Metabolic Diseases, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.

PMID: 26068998
DOI: 10.1002/9780470942390.mo110118

Abstract

Atherosclerosis is characterized by excessive cholesterol accumulation in the vessel wall. Current therapies mainly aim at decreasing influx through lowering plasma LDL-cholesterol levels. The challenge is to develop therapeutic interventions to increase efflux of excess cholesterol from the vessel wall. The pathway that mediates this efflux from vessel wall to final excretion in the feces is called reverse cholesterol transport. Recently, it has become apparent that the intestine plays an important regulatory role in this pathway. This article describes in detail a variety of experimental approaches to measure cholesterol fluxes in the hepatobiliary system as well as in the intestinal pathway. Curr. Protoc. Mouse Biol. 1:413-427 © 2011 by John Wiley & Sons, Inc.

Keywords: biliary excretion; excretion; fecal cholesterol excretion; mouse; transintestinal cholesterol.