Transmitted Extended-Spectrum Extensively Drug-Resistant Tuberculosis in Beijing, China, with Discordant Whole-Genome Sequencing Analysis Results

Hao Li; Masood ur Rehman Kayani; Yunting Gu; Xiaobo Wang; Ting Zhu; Hongfei Duan; Yifeng Ma; Hairong Huang; Babak Javid

doi:10.1128/JCM.00891-15

Transmitted Extended-Spectrum Extensively Drug-Resistant Tuberculosis in Beijing, China, with Discordant Whole-Genome Sequencing Analysis Results

J Clin Microbiol. 2015 Aug;53(8):2781-4. doi: 10.1128/JCM.00891-15. Epub 2015 Jun 10.

Authors

Hao Li¹, Masood ur Rehman Kayani², Yunting Gu³, Xiaobo Wang³, Ting Zhu², Hongfei Duan⁴, Yifeng Ma³, Hairong Huang⁵, Babak Javid⁶

Affiliations

¹ Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases, School of Medicine, Tsinghua University, Beijing, China.
² Collaborative Innovation Centre for the Diagnosis and Treatment of Infectious Diseases, School of Life Sciences, Tsinghua University, Beijing, China.
³ National Clinical Laboratory on Tuberculosis, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing Chest Hospital, Capital Medical University, Beijing, China.
⁴ Department of Tuberculosis, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing Chest Hospital, Capital Medical University, Beijing, China.
⁵ National Clinical Laboratory on Tuberculosis, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing Chest Hospital, Capital Medical University, Beijing, China huanghairong@tb123.org bjavid@gmail.com.
⁶ Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases, School of Medicine, Tsinghua University, Beijing, China huanghairong@tb123.org bjavid@gmail.com.

Abstract

Drug resistance to tuberculosis remains a major public health threat. Here, we report two cases of extended-spectrum extensively drug-resistant (XXDR) tuberculosis showing resistance to most first- and second-line agents. The results of a correlation of whole-genome sequencing (WGS) and phenotypic testing were discordant, suggesting that overreliance on WGS may miss clinically relevant resistance in extensively drug-resistant disease.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Adult
Antitubercular Agents / pharmacology
Beijing
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Extensively Drug-Resistant Tuberculosis / diagnosis*
Extensively Drug-Resistant Tuberculosis / transmission*
Genome, Bacterial*
Genotype
Humans
Male
Microbial Sensitivity Tests
Middle Aged
Molecular Sequence Data
Mycobacterium tuberculosis / classification*
Mycobacterium tuberculosis / genetics*
Mycobacterium tuberculosis / isolation & purification
Sequence Analysis, DNA

Substances

Antitubercular Agents
DNA, Bacterial

Associated data

SRA/SRP058024