Two-Photon Probes for Lysosomes and Mitochondria: Simultaneous Detection of Lysosomes and Mitochondria in Live Tissues by Dual-Color Two-Photon Microscopy Imaging

Chem Asian J. 2015 Oct;10(10):2240-9. doi: 10.1002/asia.201500314. Epub 2015 Jul 14.

Abstract

Novel two-photon (TP) probes were developed for lysosomes (PLT-yellow) and mitochondria (BMT-blue and PMT-yellow). These probes emitted strong TP-excited fluorescence in cells at widely separated wavelength regions and displayed high organelle selectivity, good cell permeability, low cytotoxicity, and pH insensitivity. The BMT-blue and PLT-yellow probes could be utilized to detect lysosomes and mitochondria simultaneously in live tissues by using dual-color two-photon microscopy, with minimum interference from each other.

Keywords: fluorescent probes; imaging agents; lysosomes; mitochondria; two photon.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Fluorescent Dyes / chemistry
  • HeLa Cells
  • Hippocampus
  • Humans
  • Hydrogen-Ion Concentration
  • Lysosomes / chemistry
  • Lysosomes / physiology*
  • Microscopy / methods*
  • Mitochondria / chemistry
  • Mitochondria / physiology*
  • Rats
  • Staining and Labeling

Substances

  • Fluorescent Dyes