Synthesis of DNA fragments containing 2'-deoxy-4'-selenonucleoside units using DNA polymerases: comparison of dNTPs with O, S and Se at the 4'-position in replication

N Tarashima; T Sumitomo; H Ando; K Furukawa; T Ishida; N Minakawa

doi:10.1039/c5ob00941c

Synthesis of DNA fragments containing 2'-deoxy-4'-selenonucleoside units using DNA polymerases: comparison of dNTPs with O, S and Se at the 4'-position in replication

Org Biomol Chem. 2015 Jul 7;13(25):6949-52. doi: 10.1039/c5ob00941c. Epub 2015 Jun 5.

Authors

N Tarashima¹, T Sumitomo, H Ando, K Furukawa, T Ishida, N Minakawa

Affiliation

¹ Graduate School of Pharmaceutical Sciences, Tokushima University, Shomachi 1-78-1, Tokushima 770-8505, Japan. minakawa@tokushima-u.ac.jp.

PMID: 26053864
DOI: 10.1039/c5ob00941c

Abstract

4'-SelenoDNA fragments were synthesized for the first time using 4'-selenothymidine triphosphate (SeTTP) by taking advantage of its bioequivalence against DNA polymerases. DNA fragments each with a homologous element (O, S or Se) at the 4'-position of the thymidine units were effectively amplified using KOD Dash DNA polymerase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
DNA / chemistry*
DNA / metabolism
DNA Replication
DNA-Directed DNA Polymerase / metabolism
Models, Molecular
Organoselenium Compounds / chemistry*
Organoselenium Compounds / metabolism
Polymerase Chain Reaction
Thymidine / analogs & derivatives*
Thymidine / chemistry
Thymidine / metabolism

Substances

4'-selenothymidine
Organoselenium Compounds
DNA
DNA-Directed DNA Polymerase
Thymidine