Effect on vascular permeability of a self-etching adhesive system containing an antimicrobial quaternary ammonium polymer QAMP into subcutaneous tissue of rats

Abstract

Objective: This study investigated the effect of a self-etching adhesive system containing an antimicrobial quaternary ammonium methacrylate polymer (QAMP) on the vascular permeability using Evans blue and laser-Doppler flowmetric methods.

Design: Forty rats were anesthetized and divided into groups: saline solution; Clearfil™ Protect Bond; Clearfil™ SE Bond; Clearfil™ SE Bond containing QAMP. Injections of Evans blue were administrated intravenously and the substances were injected intradermally. Immediately before injection and after 3 and 6h, was evaluated the blood flow. The animals were killed 3 and 6h after injection. The dorsal skin was dissected and experimental sites were obtained. The vascular permeability was evaluated by dye extravasation area, the dye was extracted and absorbance measured.

Results: Concerning the Evans blue method, Clearfil™ SE Bond containing QAMP showed an extravasation area statistically similar to Clearfil™ SE Bond and lower than Clearfil™ Protect Bond. No statistical difference was verified among experimental groups for the amount of dye extracted. Clearfil™ SE Bond containing QAMP provided better laser-Doppler flowmetric parameters than Clearfil™ Protect Bond immediately and 3h after injection.

Conclusion: The QAMP had lower effect on the exudative phase of the inflammatory process.

Keywords: Acute-phase reaction; Evans blue; Inflammation; Laser-Doppler flowmetry; Quaternary ammonium compounds.