C60 fullerene prevents genotoxic effects of doxorubicin in human lymphocytes in vitro

Ukr Biochem J. 2015 Jan-Feb;87(1):91-8.

Abstract

The self-ordering of C60 fullerene, doxorubicin and their mixture precipitated from aqueous solutions was investigated using atomic-force microscopy. The results suggest the complexation between the two compounds. The genotoxicity of doxorubicin in complex with C60 fullerene (C 60+Dox) was evaluated in vitro with comet assay using human lymphocytes. The obtained results show that the C60 fullerene prevents the toxic effect of Dox in normal cells and, thus, C60+Dox complex might be proposed for biomedical application.

MeSH terms

  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacology*
  • Cell Survival / drug effects
  • Comet Assay
  • DNA Fragmentation / drug effects
  • Doxorubicin / chemistry
  • Doxorubicin / pharmacology*
  • Drug Carriers / chemistry*
  • Fullerenes / chemistry*
  • Humans
  • Lymphocytes / cytology
  • Lymphocytes / drug effects*
  • Microscopy, Atomic Force
  • Mutagenicity Tests
  • Primary Cell Culture
  • Surface Properties

Substances

  • Antineoplastic Agents
  • Drug Carriers
  • Fullerenes
  • Doxorubicin
  • fullerene C60