Structure of Slitrk2-PTPδ complex reveals mechanisms for splicing-dependent trans-synaptic adhesion

Atsushi Yamagata; Yusuke Sato; Sakurako Goto-Ito; Takeshi Uemura; Asami Maeda; Tomoko Shiroshima; Tomoyuki Yoshida; Shuya Fukai

doi:10.1038/srep09686

Structure of Slitrk2-PTPδ complex reveals mechanisms for splicing-dependent trans-synaptic adhesion

Sci Rep. 2015 May 19:5:9686. doi: 10.1038/srep09686.

Authors

Atsushi Yamagata¹, Yusuke Sato¹, Sakurako Goto-Ito², Takeshi Uemura³, Asami Maeda², Tomoko Shiroshima², Tomoyuki Yoshida⁴, Shuya Fukai¹

Affiliations

¹ 1] Structural Biology Laboratory, Life Science Division, Synchrotron Radiation Research Organization and Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan [2] Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8501, Japan [3] CREST, JST, Saitama 332-0012, Japan.
² 1] Structural Biology Laboratory, Life Science Division, Synchrotron Radiation Research Organization and Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan [2] CREST, JST, Saitama 332-0012, Japan.
³ 1] CREST, JST, Saitama 332-0012, Japan [2] Department of Molecular and Cellular Physiology, Shinshu University School of Medicine, Nagano 390-8621, Japan [3] Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Nagano 390-8621, Japan.
⁴ 1] Department of Molecular Neuroscience, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan [2] PRESTO, JST, Saitama 332-0012, Japan.

Abstract

Selective binding between pre- and postsynaptic adhesion molecules can induce synaptic differentiation. Here we report the crystal structure of a synaptogenic trans-synaptic adhesion complex between Slit and Trk-like family member 2 (Slitrk2) and receptor protein tyrosine phosphatase (RPTP) δ. The structure and site-directed mutational analysis revealed the structural basis of splicing-dependent adhesion between Slitrks and type IIa RPTPs for inducing synaptic differentiation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Binding Sites
Humans
Mice
Models, Molecular*
Protein Binding
Protein Conformation*
Protein Interaction Domains and Motifs
Receptor-Like Protein Tyrosine Phosphatases, Class 2 / chemistry*
Receptor-Like Protein Tyrosine Phosphatases, Class 2 / genetics
Receptor-Like Protein Tyrosine Phosphatases, Class 2 / metabolism
Repressor Proteins / chemistry
Repressor Proteins / metabolism
Structure-Activity Relationship
Synapses / physiology*

Substances

LRR1 protein, human
Repressor Proteins
Ptprd protein, mouse
Receptor-Like Protein Tyrosine Phosphatases, Class 2

Associated data

PDB/4Y61