Molecular cloning and characterization of annexin genes in peanut (Arachis hypogaea L.)

MeiJing He; XinLei Yang; ShunLi Cui; GuoJun Mu; MingYu Hou; HuanYing Chen; LiFeng Liu

doi:10.1016/j.gene.2015.05.004

Molecular cloning and characterization of annexin genes in peanut (Arachis hypogaea L.)

Gene. 2015 Aug 15;568(1):40-9. doi: 10.1016/j.gene.2015.05.004. Epub 2015 May 7.

Authors

MeiJing He¹, XinLei Yang¹, ShunLi Cui¹, GuoJun Mu¹, MingYu Hou¹, HuanYing Chen¹, LiFeng Liu²

Affiliations

¹ North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding 071001, People's Republic of China.
² North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding 071001, People's Republic of China. Electronic address: lifengliucau@126.com.

PMID: 25958350
DOI: 10.1016/j.gene.2015.05.004

Abstract

Annexin, Ca(2+) or phospholipid binding proteins, with many family members are distributed throughout all tissues during plant growth and development. Annexins participate in a number of physiological processes, such as exocytosis, cell elongation, nodule formation in legumes, maturation and stress response. Six different full-length cDNAs and two partial-length cDNAs of peanut, (AnnAh1, AnnAh2, AnnAh3, AnnAh5, AnnAh6, AnnAh7, AnnAh4 and AnnAh8) encoding annexin proteins, were isolated and characterized using a RT-PCR/RACE-PCR based strategy. The predicted molecular masses of these annexins were 36.0kDa with acidic pIs of 5.97-8.81. ANNAh1, ANNAh2, ANNAh3, ANNAh5, ANNAh6 and ANNAh7 shared sequence similarity from 35.76 to 66.35% at amino acid level. Phylogenetic analysis revealed their evolutionary relationships with corresponding orthologous sequences in soybean and deduced proteins in various plant species. Real-time quantitative assays indicated that these genes were differentially expressed in various organs. Transcript level analysis for six annexin genes under stress conditions showed that these genes were regulated by drought, salinity, heavy metal stress, low temperature and hormone. Additionally, the prediction of cis-regulatory element suggested that different cis-responsive elements including stress- and hormone-responsive-related elements could respond to various stress conditions. These results indicated that members of AnnAhs family may play important roles in the adaptation of peanut to various environmental stresses.

Keywords: Annexin genes; Cloning; Expression analysis; Peanut.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Annexins / genetics*
Arachis / genetics*
Base Sequence
Cloning, Molecular
Gene Expression
Gene Expression Regulation, Plant
Molecular Sequence Data
Phylogeny
Plant Proteins / genetics*
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Stress, Physiological

Substances

Annexins
Plant Proteins