Helicobacter pylori vesicles carrying CagA localize in the vicinity of cell-cell contacts and induce histone H1 binding to ATP in epithelial cells

FEMS Microbiol Lett. 2015 Jun;362(11):fnv076. doi: 10.1093/femsle/fnv076. Epub 2015 May 7.

Abstract

Helicobacter pylori produces outer membrane vesicles (OMV), delivering bacterial substances including the oncogenic cytotoxin-associated CagA protein to their surroundings. We investigated the effects of H. pylori OMV carrying CagA (OMV-CagA) on cell junctions and ATP-binding proteome of epithelial monolayers, using proteomics, mass spectrometry and imaging. OMV-CagA localized in close vicinity of ZO-1 tight junction protein and induced histone H1 binding to ATP. We suggest the expression of novel events in the interactions between H. pylori OMV and epithelia, which may have an influence on host gene transcription and lead to different outcomes of an infection and development of cancer.

Keywords: ATP-proteome; CagA; Helicobacter pylori; epithelial cell-cell junctions; histone H1; outer membrane vesicles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / metabolism*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Caco-2 Cells
  • Helicobacter pylori / genetics
  • Helicobacter pylori / metabolism*
  • Histones / metabolism*
  • Host-Pathogen Interactions
  • Humans
  • Microscopy, Confocal
  • Tight Junctions / metabolism
  • Tight Junctions / ultrastructure
  • Zonula Occludens-1 Protein / metabolism

Substances

  • Antigens, Bacterial
  • Bacterial Proteins
  • Histones
  • Zonula Occludens-1 Protein
  • cagA protein, Helicobacter pylori
  • Adenosine Triphosphate