Efficient construction of stable gene nanoparticles through polymerase chain reaction with flexible branched primers for gene delivery

Jianbing Liu; Runyu Wang; Dejun Ma; Di Ouyang; Zhen Xi

doi:10.1039/c5cc01788b

Efficient construction of stable gene nanoparticles through polymerase chain reaction with flexible branched primers for gene delivery

Chem Commun (Camb). 2015 Jun 4;51(44):9208-11. doi: 10.1039/c5cc01788b.

Authors

Jianbing Liu¹, Runyu Wang, Dejun Ma, Di Ouyang, Zhen Xi

Affiliation

¹ Department of Chemical Biology, State Key Laboratory of Elemento-Organic Chemistry, National Engineering Research Center of Pesticide (Tianjin), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, China. zhenxi@nankai.edu.cn.

PMID: 25952052
DOI: 10.1039/c5cc01788b

Abstract

Flexible branched primers were designed to construct stable gene nanoparticles with multiple target gene copies through polymerase chain reaction, which can be used as an efficient transcription template in eukaryotic cells for gene delivery.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cytomegalovirus / genetics
DNA / chemistry*
DNA Primers
Gene Transfer Techniques*
Green Fluorescent Proteins / genetics
HEK293 Cells
Humans
Nanoparticles / chemistry*
Polymerase Chain Reaction

Substances

DNA Primers
enhanced green fluorescent protein
Green Fluorescent Proteins
DNA