Abstract
This study was focused on evaluation of the cytotoxicity and apoptotic affects of benzofuran derivative on MCF-7 breast cancer cell line. This effective compound was isolated from the root of Petasites hybridus plant. For experiments, the MCF-7 cells were treated with several concentrations (0-500μM) of 1-(6-hydroxy-2- isopropenyl-1-benzofuran-5-yl)-1-ethanone 1 at different times. In this study, test of neutral red was also employed for cytotoxicity assay and quantity of P53, P21. Bax genes expression was analyzed using Real-Time PCR and ELISA techniques. Results show that compound 1 has cytotoxicity and apoptotic effects on Human breast cancer (Michigan Cancer Foundation-7) MCF-7 cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents, Phytogenic / chemistry
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Antineoplastic Agents, Phytogenic / isolation & purification
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Antineoplastic Agents, Phytogenic / pharmacology*
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Apoptosis / drug effects*
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Benzofurans / chemistry
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Benzofurans / isolation & purification
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Benzofurans / pharmacology*
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Biological Products / chemistry
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Biological Products / isolation & purification
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Biological Products / pharmacology*
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Breast Neoplasms / pathology*
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Cell Proliferation / drug effects
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Dose-Response Relationship, Drug
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Drug Screening Assays, Antitumor
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Humans
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MCF-7 Cells
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Molecular Structure
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Petasites / chemistry
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Plant Roots / chemistry
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Structure-Activity Relationship
Substances
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Antineoplastic Agents, Phytogenic
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Benzofurans
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Biological Products
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benzofuran