Molybdopterin biosynthesis-Mechanistic studies on a novel MoaA catalyzed insertion of a purine carbon into the ribose of GTP

Biochim Biophys Acta. 2015 Sep;1854(9):1073-7. doi: 10.1016/j.bbapap.2015.04.008. Epub 2015 Apr 17.

Abstract

The first step in the biosynthesis of the molybdopterin cofactor involves an unprecedented insertion of the purine C8 carbon between the C2' and C3' carbons of the ribose moiety of GTP. Here we review mechanistic studies on this remarkable transformation. This article is part of a Special Issue entitled: Cofactor-dependent proteins: evolution, chemical diversity and bio-applications.

Keywords: Intermediate trapping; Mechanism; MoaA; Molybtopterin; Substrate analog.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Biocatalysis*
  • Carbon
  • Coenzymes / biosynthesis*
  • Escherichia coli Proteins / physiology*
  • Guanosine Triphosphate / chemistry*
  • Humans
  • Isomerases / physiology*
  • Metalloproteins / biosynthesis*
  • Molybdenum Cofactors
  • Pteridines
  • Purines / chemistry*
  • Ribose / chemistry*

Substances

  • Coenzymes
  • Escherichia coli Proteins
  • Metalloproteins
  • Molybdenum Cofactors
  • Pteridines
  • Purines
  • Ribose
  • Carbon
  • Guanosine Triphosphate
  • molybdenum cofactor
  • Isomerases
  • MoaA protein, E coli
  • purine