Abstract
Background:
To determine the antiproliferative effect of gamma-tocotrienol (GTT) treatment on differential protein expression in HepG2 cells.
Methods:
HepG2 cells were treated with 70 μM GTT for 48 hours and differentially expressed protein spots were determined by two-dimensional electrophoresis (2DE), identified by MALDI-TOF mass spectrometer (MS) and validated by quantitative real-time polymerase chain reaction (qRT-PCR).
Results:
GTT treatment on HepG2 cells showed a total of five differentially expressed proteins when compared to their respective untreated cells where three proteins were down-regulated and two proteins were up-regulated. One of these upregulated proteins was identified as peroxiredoxin-4 (Prx4). Validation by qRT-PCR however showed decreased expression of Prx4 mRNA in HepG2 cells following GTT treatment.
Conclusions:
GTT might directly influence the expression dynamics of peroxiredoxin-4 to control proliferation in liver cancer.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Antineoplastic Agents / pharmacology*
-
Antineoplastic Agents / therapeutic use
-
Antioxidants / pharmacology*
-
Antioxidants / therapeutic use
-
Bile Duct Neoplasms
-
Chromans / metabolism
-
Down-Regulation
-
Electrophoresis, Gel, Two-Dimensional
-
Hep G2 Cells
-
Hepatoblastoma / drug therapy
-
Hepatoblastoma / metabolism*
-
Humans
-
Liver / drug effects*
-
Liver / metabolism
-
Liver Neoplasms / drug therapy
-
Liver Neoplasms / metabolism*
-
Peroxiredoxins / metabolism*
-
Proteins / metabolism
-
Proteomics
-
RNA, Messenger / metabolism
-
Real-Time Polymerase Chain Reaction
-
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
-
Tocotrienols / pharmacology*
-
Tocotrienols / therapeutic use
-
Up-Regulation
-
Vitamin E / analogs & derivatives
-
Vitamin E / metabolism
-
Vitamins / pharmacology
-
Vitamins / therapeutic use
Substances
-
Antineoplastic Agents
-
Antioxidants
-
Chromans
-
Proteins
-
RNA, Messenger
-
Tocotrienols
-
Vitamins
-
Vitamin E
-
plastochromanol 8
-
Peroxiredoxins