Levels in plasma of the serine proteases and associated proteins of the lectin pathway are altered in patients with systemic lupus erythematosus

Anne Troldborg; Steffen Thiel; Magdalena Janina Laska; Bent Deleuran; Jens Christian Jensenius; Kristian Stengaard-Pedersen

doi:10.3899/jrheum.141163

Levels in plasma of the serine proteases and associated proteins of the lectin pathway are altered in patients with systemic lupus erythematosus

J Rheumatol. 2015 Jun;42(6):948-51. doi: 10.3899/jrheum.141163. Epub 2015 Apr 15.

Authors

Anne Troldborg¹, Steffen Thiel², Magdalena Janina Laska², Bent Deleuran², Jens Christian Jensenius², Kristian Stengaard-Pedersen²

Affiliations

¹ From the Department of Rheumatology, Aarhus University Hospital; Department of Clinical Medicine, and Department of Biomedicine, Aarhus University, Aarhus, Denmark.A. Troldborg, MD, PhD candidate, Department of Rheumatology, Aarhus University Hospital and Department of Clinical Medicine, Aarhus University; S. Thiel, Professor; M.J. Laska, PhD, Assistant Professor, Department of Biomedicine, Aarhus University; B. Deleuran, DrMed, Professor, Department of Rheumatology, Aarhus University Hospital and Department of Biomedicine, Aarhus University; J.C. Jensenius, DrMed, DrPhil, Professor, Department of Biomedicine, Aarhus University; K. Stengaard-Pedersen, DrMed, Professor, Department of Rheumatology, Aarhus University Hospital and Department of Clinical Medicine, Aarhus University. annetrol@rm.dk.
² From the Department of Rheumatology, Aarhus University Hospital; Department of Clinical Medicine, and Department of Biomedicine, Aarhus University, Aarhus, Denmark.A. Troldborg, MD, PhD candidate, Department of Rheumatology, Aarhus University Hospital and Department of Clinical Medicine, Aarhus University; S. Thiel, Professor; M.J. Laska, PhD, Assistant Professor, Department of Biomedicine, Aarhus University; B. Deleuran, DrMed, Professor, Department of Rheumatology, Aarhus University Hospital and Department of Biomedicine, Aarhus University; J.C. Jensenius, DrMed, DrPhil, Professor, Department of Biomedicine, Aarhus University; K. Stengaard-Pedersen, DrMed, Professor, Department of Rheumatology, Aarhus University Hospital and Department of Clinical Medicine, Aarhus University.

PMID: 25877499
DOI: 10.3899/jrheum.141163

Abstract

Objective: To examine whether proteins of the lectin pathway of the complement system are involved in systemic lupus erythematosus (SLE) pathogenesis.

Methods: Using time-resolved immunofluorometric assays, plasma levels of mannan-binding lectin (MBL)-associated serine proteases 1 (MASP-1), MASP-2, MASP-3, MBL-associated protein of 19 kDa (MAp19), and MAp44 were determined in 58 patients with SLE and 65 healthy controls (HC).

Results: Plasma concentrations in patients with SLE were higher than HC regarding MASP-1, MASP-3, and MAp44 (p < 0.0001, 0.0003, and 0.0013). Complement factor 3 correlated negatively and anti-dsDNA positively with levels of MAp19 (p = 0.0035, p = 0.0133).

Conclusion: In SLE, plasma levels of MASP and MAp are altered and associated with SLE characteristics, supporting a role in SLE pathogenesis.

Keywords: COMPLEMENT SYSTEM; INNATE IMMUNITY; LECTIN PATHWAY; SYSTEMIC LUPUS ERYTHEMATOSUS.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
Cohort Studies
Complement System Proteins / metabolism
Cross-Sectional Studies
Denmark
Disease Progression
Female
Humans
Immunity, Innate / immunology*
Logistic Models
Lupus Erythematosus, Systemic / blood
Lupus Erythematosus, Systemic / immunology*
Lupus Erythematosus, Systemic / physiopathology*
Mannose-Binding Lectin / genetics
Mannose-Binding Lectin / metabolism*
Mannose-Binding Protein-Associated Serine Proteases / metabolism*
Middle Aged
Sensitivity and Specificity
Serine Proteases / blood
Serine Proteases / metabolism
Severity of Illness Index
Signal Transduction
Statistics, Nonparametric
Young Adult

Substances

Mannose-Binding Lectin
Complement System Proteins
Serine Proteases
Mannose-Binding Protein-Associated Serine Proteases