Polyphenols as possible bioprotectors against cytotoxicity and DNA damage induced by ochratoxin A

Environ Toxicol Pharmacol. 2015 May;39(3):1008-18. doi: 10.1016/j.etap.2015.03.013. Epub 2015 Mar 28.

Abstract

The present study aimed to investigate the protective effects of luteolin (L), chlorogenic acid (ChlA) and caffeic acid (CafA) against cyto-genotoxic effects caused by OTA. Vero cells and rat lymphocytes were used and viability was measured by neutral red uptake, MTT and trypan blue dye exclusion method. L (50 and 100μg/mL), ChlA (100 and 200μg/mL) and CafA (10-50μg/mL) reduced the damage induced by OTA (10μg/mL) on both cells type shown a good protective effect. The comet and micronucleus tests in Balb/c mice were performed. ChlA (10mg/kg bw) reduced OTA (0.85mg/kg bw)-induced DNA damage on blood and bone marrow cells, CafA (10mg/kg bw) showed protective effect only in blood cells and luteolin (2.5mg/kg bw) failed to protect DNA integrity on cells. In conclusion, polyphenols tested reduced the toxicity caused by OTA on different target cells with good protective effect, being ChlA the compound that showed the best effects.

Keywords: Caffeic acid; Chlorogenic acid; Cytotoxicity; DNA damage; Luteolin; Ochratoxin A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Caffeic Acids / pharmacology
  • Carcinogens / toxicity*
  • Cell Survival / drug effects
  • Chlorocebus aethiops
  • Chlorogenic Acid / pharmacology
  • DNA Damage / drug effects*
  • Luteolin / pharmacology
  • Lymphocytes / drug effects
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Ochratoxins / toxicity*
  • Organ Specificity
  • Polyphenols / pharmacology*
  • Rats
  • Vero Cells

Substances

  • Antioxidants
  • Caffeic Acids
  • Carcinogens
  • Ochratoxins
  • Polyphenols
  • ochratoxin A
  • Chlorogenic Acid
  • Luteolin
  • caffeic acid