Y chromosomal haplotype characteristics of domestic sheep (Ovis aries) in China

Yutao Wang; Lei Xu; Wei Yan; Shaobin Li; Jiqing Wang; Xiu Liu; Jiang Hu; Yuzhu Luo

doi:10.1016/j.gene.2015.04.015

Y chromosomal haplotype characteristics of domestic sheep (Ovis aries) in China

Gene. 2015 Jul 10;565(2):242-5. doi: 10.1016/j.gene.2015.04.015. Epub 2015 Apr 9.

Authors

Yutao Wang¹, Lei Xu², Wei Yan², Shaobin Li², Jiqing Wang², Xiu Liu², Jiang Hu², Yuzhu Luo³

Affiliations

¹ Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; The Key Laboratory of Ecology and Biological Resources in Yarkand Oasis at Colleges & Universities under the Department of Education of Xinjiang Uygur Autonomous Region, Kashgar Teachers College, Kashgar 844000, China.
² Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
³ Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China. Electronic address: luoyz@gsau.edu.cn.

PMID: 25865303
DOI: 10.1016/j.gene.2015.04.015

Abstract

Investigations on the variation present at the male-specific Y chromosome region provide strong information to understand the origin and evolution of domestic sheep. One SNP OY1 (g.88A>G) in the upstream region of SRY gene, and the microsatellite SRYM18 locus within ovine Y chromosome were analyzed in one hundred and forty five samples collected from eleven breeds in China. SNP OY1 was analyzed using PCR-SSCP method and sequencing. Two different PCR-SSCP patterns represented two specific sequences with sequence analysis revealing SNP-OY1 (g.88A>G) were observed, while SNP A-OY1 showed the most common frequency (82.8%). Sequencing of the SRYM18 region revealed one novel size fragment (A2) with different repetitive units. Seven haplotypes (H4, H5, H6, H7, H8, H9 and H12) and two novel haplotypes (Ha and Hb) were established using combined genotype analysis. H6 showed the highest frequency (43.4%) across all breeds, and H8 showed the second frequency (24.1%). Ha was only found in one breed (Tan), while Hb was present in three breeds (Gansu alpine, White Suffolk and Duolang). Our findings reveal one novel allele in SRYM18 region and two novel male haplotypes of domestic sheep in China.

Keywords: Domestic sheep; Haplotype; Microsatellite; Y chromosome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Animals
China
Haplotypes / genetics*
Male
Microsatellite Repeats / genetics
Polymorphism, Single Nucleotide / genetics
Sheep, Domestic / genetics*
Y Chromosome / genetics*