Abstract
Heme plays pivotal roles in various cellular processes as well as in iron homeostasis in living systems. Here, we report a genetically encoded fluorescence resonance energy transfer (FRET) sensor for selective heme imaging by employing a pair of bacterial heme transfer chaperones as the sensory components. This heme-specific probe allows spatial-temporal visualization of intracellular heme distribution within living cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacillus anthracis / genetics
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Bacillus anthracis / metabolism
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Biosensing Techniques / methods*
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Cell Line
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Cell Line, Tumor
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Cricetinae
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Fluorescence Resonance Energy Transfer / methods*
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Genetic Engineering
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Heme / analysis*
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Heme / metabolism
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Humans
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Mice
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Optical Imaging / methods
Substances
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Bacterial Proteins
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Luminescent Proteins
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Heme