Intron targeting-mediated and endogenous gene integrity-maintaining knockin in zebrafish using the CRISPR/Cas9 system

Affiliations

• ¹ Institute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China.
• ² 1] Institute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China [2] Graduate School, University of Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China.
• ³ 1] Institute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China [2] Drug Discovery Laboratory, School of Pharmacy, Shanghai University of Traditional Chinese Medicine, 1200 Cai-Lun Road, Shanghai 201203, China.
• ⁴ School of Life Science and Technology, ShanghaiTech University, 319 Yue-Yang Road, Shanghai 200031, China.
• ⁵ 1] Institute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China [2] Graduate School, University of Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China [3] School of Life Science and Technology, ShanghaiTech University, 319 Yue-Yang Road, Shanghai 200031, China.