In vivo induction of T-follicular helper cells by modulation of regulatory T cell function

James Badger Wing; Shimon Sakaguchi

doi:10.1007/978-1-4939-2498-1_7

In vivo induction of T-follicular helper cells by modulation of regulatory T cell function

Methods Mol Biol. 2015:1291:77-85. doi: 10.1007/978-1-4939-2498-1_7.

Authors

James Badger Wing¹, Shimon Sakaguchi

Affiliation

¹ Laboratory of Experimental Immunology, WPI Immunology Frontier Research Center, Osaka University, 3-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.

PMID: 25836303
DOI: 10.1007/978-1-4939-2498-1_7

Abstract

Regulatory T cells, particularly their follicular resident subset, T-follicular regulatory cells, play a critical role in the maintenance of immune homeostasis and prevention of the expansion of T-follicular helper cells. One of the key mechanisms used by these cells is CTLA-4 dependent suppression of inflammation. In this chapter, we detail methods to increase the formation of T-follicular helper cells following vaccination by either depleting Treg cells, via Foxp3 linked diphtheria toxin receptor, or blocking CTLA-4 function by the use of antibodies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Blocking / pharmacology
CTLA-4 Antigen / immunology
Cell Proliferation
Immunologic Techniques / methods*
Lymphocyte Depletion
Mice
Staining and Labeling
T-Lymphocytes, Helper-Inducer / cytology
T-Lymphocytes, Regulatory / cytology

Substances

Antibodies, Blocking
CTLA-4 Antigen